New Blood Test for Celiac Disease Will Eliminate Need for Gluten Challenge and Intestinal Biopsy

September 23, 2015 Researchers in the Faculty of Medicine at University of Oslo have developed a blood test that simplifies celiac disease diagnosis by Thomas Olafsen.

While consuming gluten-containing foods, you must take a blood test followed by a tissue sample from the small intestine. If you have embarked on a gluten-free diet, you must begin to consume gluten-containing foods again. If you do have coeliac disease, this may result in pain and considerable discomfort.

A diagnosis of coeliac disease also requires a tissue sample from the small intestine. During a procedure known as a gastroscopy, a tube is inserted passed your throat into the duodenum. Tissue samples must be taken in order to obtain a definite diagnosis. The procedure can be intimidating if not challenging for adults;  children are sedated during the examination.

A Misunderstanding by the Body’s Immune System

Postdoctoral fellow Asbjørn Christophersen explains, “When the food that you eat enters the small intestine, it is reduced to tiny fractions and presented to the T cells on so-called HLA molecules. The HLA molecules present various elements of what you consume, as well as what is inside the cells. The task of the T cells is to monitor cells to see if they are infected by viruses or bacteria. In the case of coeliac disease, the T cells think that gluten is a virus or bacteria. The T cells send a message to the other immune cells to attack not only the gluten protein itself, but also cells, and an enzyme that binds itself to gluten,” Christophersen further explains. Thus your small intestine becomes inflamed.”

Researchers at the Faculty have now developed a new blood test that makes it much simpler to diagnose coeliac disease.

As soon as the test is approved and released on the market, you will in principle be able to take a blood test at your GP’s surgery. The blood sample will then be sent to a laboratory which performs the test.

A reagent composed of HLA molecules and fractions of gluten is added to the sample. A reagent is a substance to which something is added to detect the presence of another substance. In this case the reagent binds itself to the T cells that are in the blood sample. Magnetized antibodies are also added which in turn bind to the reagent.

“When we allow blood cells to flow through a magnetic column, the cells that react to gluten remain suspended in the column while all the other cells flow through it. We observe that coeliacs have a much higher number of gluten-reactive T cells in their blood than non-coeliacs. The level is more or less independent of how much gluten they eat,” says Christophersen.

This means that we can diagnose coeliac disease irrespective of whether you are consuming foods containing gluten or not. You can thereby avoid the discomfort that may result from the intake of gluten, not to mention that you avoid the gastroscopy. Diagnosis becomes more effective as well as less unpleasant for the patient.

Building on existing research

The method for detecting cells that react to gluten in the blood is based on a method developed by the group at the Marc Jenkins Lab using mice. The group succeeded in studying rare T cells with very low frequency. Professor Marc Jenkins is the Director of the Center for Immunology at the University of Minnesota. “We have slightly altered the method and use it on humans. We are the first to see the potential of its use for determining a disease,” Asbjørn Christophersen explains.

Large-scale clinical trial

The researchers are now conducting a large-scale clinical trial with three groups: coeliacs, healthy individuals and persons with symptoms similar to coeliac disease. It is important to include the group with symptoms similar to coeliac disease because many of these patients will take the same test. This will also help to show whether these latter patients have signs of a T-cell reaction to gluten such as we observe in the case of coeliac disease.

Christophersen hopes to have the test available on the market within a few years:

“We are now in contact with several leading international companies that are interested in using the technique for the diagnosis of celiac disease,” he says.

The technique can also play a part in developing a vaccine for coeliac disease. The purpose of a vaccine is for the person receiving it to develop so-called regulatory T cells. These are T cells which can suppress the immune system’s unwanted response to gluten.

“We are also working to optimize the method further,” says Asbjørn Christophersen.

 The original item was written by Thomas Olafsen https://www.med.uio.no/klinmed/english/research/news-and-events/news/2015/simplified-diagnosis-coeliac.html